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KMID : 0374019960190010067
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Ewha Medical Journal
1996 Volume.19 No. 1 p.67 ~ p.72
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A Study of in situ PCR on Borderline Leprosy Patients
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Abstract
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This study was planned to help the diagnosis of borderline leprosy by application of the
insitu PCR. In situ PCR was performed in the tissue from skin biopsy using the Dig DNA
probe synthesis kit@(Behringer Mannheim Co) on silanized slides and Dig incorporated PCR
products were visualized by alkaline phosphatase conjugated anti-Dig antibody using
NBT/BCIP as substrate. In the borderline lepromatous patients homogeneous or granular
strong deposits were observed mainly within the cytoplasm of the histiocytes and proliferated
Schwann cells. Some secretory cells of sweat glands, erector pile muscle and a few
endothelial cells were weakly stained. In the tuberculoid leprosy patients no positive signal
was observed. In conclusion in situ PCR can be applied on the tissue of berderline
lepromatous patients of which sample does not need proteinase K treatment.
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KEYWORD
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